antibodies against cxcl1 Search Results


antibody against cxcl1  (Biozol Diagnostica Vertrieb GmbH)
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Biozol Diagnostica Vertrieb GmbH antibody against cxcl1
a) Gene expression values of <t>CXCL1</t> in normal skin (NS), normal human melanocytes (NHM), melanomas in situ (MIS), primary (PM) and metastatic (MM) melanomas were obtained from oligonucleotide microarray data . The expression values of single samples (symbols) and the mean expression level (lines) are reported. b) CXCL1 expression during progression from normal human melanocytes (1) to melanoma in situ (2), primary (3) and metastatic (4) melanoma. There is a significant trend to increased CXCL1 expression during melanoma progression. c) qRT-PCR analysis of M14 melanoma cells shows that treatment with Curcumin for several time periods (2, 4, 6, 15 and 24 h) did not effect CXCL1 expression. d) Well in line with RT-PCR results, CXCL1 expression visualized here by Western Blots of cell culture supernatants was not altered in M14 cells treated for several time intervals (6 h, 15 h, 24 h) with Curcumin (lanes indicated with
Antibody Against Cxcl1, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a) Gene expression values of CXCL1 in normal skin (NS), normal human melanocytes (NHM), melanomas in situ (MIS), primary (PM) and metastatic (MM) melanomas were obtained from oligonucleotide microarray data . The expression values of single samples (symbols) and the mean expression level (lines) are reported. b) CXCL1 expression during progression from normal human melanocytes (1) to melanoma in situ (2), primary (3) and metastatic (4) melanoma. There is a significant trend to increased CXCL1 expression during melanoma progression. c) qRT-PCR analysis of M14 melanoma cells shows that treatment with Curcumin for several time periods (2, 4, 6, 15 and 24 h) did not effect CXCL1 expression. d) Well in line with RT-PCR results, CXCL1 expression visualized here by Western Blots of cell culture supernatants was not altered in M14 cells treated for several time intervals (6 h, 15 h, 24 h) with Curcumin (lanes indicated with

Journal: Molecular Cancer

Article Title: Overexpression of the ATP binding cassette gene ABCA1 determines resistance to Curcumin in M14 melanoma cells

doi: 10.1186/1476-4598-8-129

Figure Lengend Snippet: a) Gene expression values of CXCL1 in normal skin (NS), normal human melanocytes (NHM), melanomas in situ (MIS), primary (PM) and metastatic (MM) melanomas were obtained from oligonucleotide microarray data . The expression values of single samples (symbols) and the mean expression level (lines) are reported. b) CXCL1 expression during progression from normal human melanocytes (1) to melanoma in situ (2), primary (3) and metastatic (4) melanoma. There is a significant trend to increased CXCL1 expression during melanoma progression. c) qRT-PCR analysis of M14 melanoma cells shows that treatment with Curcumin for several time periods (2, 4, 6, 15 and 24 h) did not effect CXCL1 expression. d) Well in line with RT-PCR results, CXCL1 expression visualized here by Western Blots of cell culture supernatants was not altered in M14 cells treated for several time intervals (6 h, 15 h, 24 h) with Curcumin (lanes indicated with "+") versus respective un-treated (lanes indicated with "-") cells. Equal amounts of total protein were applied per lane and experiments were performed in triplicate.

Article Snippet: Conditioned media from Curcumin-treated (6, 15 and 24 h) and non-treated control cells were analyzed using an antibody against CXCL1 (Dianova, Hamburg, Germany).

Techniques: Expressing, In Situ, Microarray, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Western Blot, Cell Culture